Invitrogen™ FAM21C Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA535883

Catalog No. PIPA535883

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission (PubMed:19922874, PubMed:20498093, PubMed:22513087, PubMed:23331060). Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35 (PubMed:24980502). Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L (PubMed:25355947, PubMed:28892079). [UniProt].

Specifications

• Antigen: FAM21C
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WASHC2C
• Gene Alias: Fam21; FAM21A; FAM21C; family with sequence similarity 21 member C; family with sequence similarity 21, member C; KIAA0592; NP61201; vaccinia virus penetration factor; VPEF; WASH complex subunit 2; WASH complex subunit 2C; WASH complex subunit FAM21; WASH complex subunit FAM21C; Washc2; WASHC2C
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 253725
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9Y4E1
• Gene Symbols: WASHC2C
• Immunogen: Recombinant fragment corresponding to a region within amino acids 87 and 305 of FAM21C.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG