MilliporeSigma™ FBXO15, Mouse, Unlabeled, Clone: 3B11.1,

Catalog No.	Quantity	Conjugate
MABD127MI	200 μL	Unconjugated

Catalog No. MABD127MI Supplier MilliporeSigma™ Supplier No. MABD127

Description

Mouse Monoclonal Antibody

F-box only protein 15 (UniProt Q8NCQ5) is encoded by the FBXO15 (also known as FBX15) gene (Gene ID 201456) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, U-box-type, and PHD-finger-type E3 ligases. The RING finger-type E3s include seven cullin-based E3s, such as SKP1–CUL1–F-box-protein (SCF) complex and the anaphase-promoting complex/cyclosome (APC/C). F-box proteins use their N-terminal F-box motif to bind Skp1 and other motif(s) in their C-terminal region for substrates recognition and recruitment. F-box proteins are divided into the FBXW subclass that contains WD40 repeat domains, the FBXL subclass that contains leucine-rich repeat domains, and the F-box only (FBXO) subclass that contains not yet fully characterized substrate recognition domains. SCF-Fbxo15 is reported to mediate the ubiquitination of cardiolipin synthase (CLS1) for proteasomal degradation in human lung epithelial cells. PTEN-induced putative kinase 1 (PINK1) is shown to phosphorylate CLS1 and cause its release from the inner mitochondrial membrane to the outer membrane, making it accessible for Fbxo15-targeted ubiquitination.

Specifications

• Antigen: FBXO15
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 3B11.1
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgMκ antibody in buffer containing PBS with 0.05% Sodium Azide.
• Gene: FBXO15, FBX15
• Gene Accession No.: Q8NCQ5
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding an N-terminal sequence of human FBXO15.
• Purification Method: Purified by Ion-Exchange Chromatography
• Quantity: 200 μL
• Regulatory Status: RUO
• Research Discipline: Stem Cell Research
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001136430
• Test Specificity: Clone 3B11.1 recoginzes an N-terminal epitope present in spliced isoform 1 (UniProt Q8NCQ5-1), but absent in spliced isoform 2 (UniProt Q8NCQ5-2).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgM κ

For Research Use Only. Not for use in diagnostic procedures.