Fc gamma RIIA/CD32a Antibody (7.3), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24472100	Novus Biologicals NBP2447210.02MG	0.02 mg
NBP24472101	Novus Biologicals NBP2447210.1MG	0.1 mg
NBP24472102	Novus Biologicals NBP2447210.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

Fc gamma RIIA/CD32a Monoclonal specifically detects Fc gamma RIIA/CD32a in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Functional, Immunofluorescence.

Specifications

• Antigen: Fc gamma RIIA/CD32a
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P12318
• Gene ID (Entrez): 2212
• Immunogen: K562 and FcgRII+L cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 40 kDa

• Clone: 7.3
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CD32 antigen, CD32A, CD32MGC23887, CDw32fc-gamma-RIIa, Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc fragment of IgG, low affinity IIa, receptor for (CD32), FCG2, Fc-gamma RII-a, Fc-gamma-RIIa, FcGR, FCGR2, FCGR2A1, FcRII-a, IGFR2MGC30032, IgG Fc receptor II-a, Immunoglobulin G Fc receptor II, low affinity immunoglobulin gamma Fc region receptor II-a
• Gene Symbols: FCGR2A
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (Fc?RII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

For Research Use Only