Invitrogen™ Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™

Polyclonal Antibody

Supplier:  Invitrogen™ 14916542

Catalog No. 50-112-2773

Description

Description: The Rhesus Fc Receptor Binding Inhibitor can be used to inhibit the non-specific Fc receptor (FcR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of rhesus tissue. FcR are expressed at varying levels in multiple cell lineages including high expression in myeloid cells, granulocytes, and B cells. The biological function of the FcR, including initiation of endocytosis, phagocytosis, and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to rhesus FcR varies depending on the isotype of the monoclonal antibody. eBioscience testing indicates that mouse IgG2a isotype is blocked by Rhesus Fc Receptor Binding Inhibitor. Applications Reported: Rhesus Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis. To inhibit the non-specific binding of mouse monoclonal antibodies add the Rhesus Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with primary antibody. Applications Tested: Rhesus Fc Receptor Binding Inhibitor has been pre-titrated and tested by flow cytometric analysis of normal rhesus peripheral blood cells. This can be used at 5 μL (10 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Purity: ...

Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.

Specifications

• Antigen: Fc Receptor Binding Inhibitor
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: Fc blocking; FcR BI; FcR Inhibitor
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Rhesus Macaque
• Product Type: Antibody
• Green Features: Responsibly packaged

• Applications: Flow Cytometry
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Host Species: Not Available
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid