Invitrogen™ FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-9417	Invitrogen™ 14589982	100 μg

Description

Description: The AER-37 monoclonal antibody reacts with the FcεRIalpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIalpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. FcεRIalpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. It has been reported to not block IgE binding. Applications Tested: The AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.

Specifications

• Antigen: FceR1 alpha
• Concentration: 0.5 mg/mL
• Applications: Flow Cytometry
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P12319
• Gene ID (Entrez): 2205
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: AER-37 (CRA1)
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2b κ
• Target Species: Human
• Gene: FCER1A
• Gene Alias: alpha polypeptide; fc epsilon r1; Fc epsilon receptor Ia; Fc epsilon RI alpha-chain; Fc fragment of IgE receptor Ia; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; Fc receptor, IgE, high affinity I, alpha polypeptide; Fce1a; Fc-epsilon RI-alpha; fcepsilonri; FCER1A; FcERI; Fcr-5; high affinity immunoglobulin epsilon receptor alpha-subunit; high affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; Iger01; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; RATIGER01; RP11-550P17.3
• Gene Symbols: FCER1A
• Product Type: Antibody