Invitrogen™ FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), NovaFluor™ Yellow 570, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
H033T02Y01A	Invitrogen™ H033T02Y01A	25 Tests
H033T03Y01A	Invitrogen™ H033T03Y01A	100 Tests

Description

Description The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested This AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood cells. This can be used at 5 μL (0.4 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here>. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining. Viability dye compatibility NovaFluor dyes are not compa...

Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.

Specifications

• Antigen: FceR1 alpha
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: NovaFluor Yellow 570
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P12319
• Gene ID (Entrez): 2205
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: AER-37 (CRA1)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2b κ
• Target Species: Human
• Gene: FCER1A
• Gene Alias: alpha polypeptide; fc epsilon r1; Fc epsilon receptor Ia; Fc epsilon RI alpha-chain; Fc fragment of IgE receptor Ia; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; Fc receptor, IgE, high affinity I, alpha polypeptide; Fce1a; Fc-epsilon RI-alpha; fcepsilonri; FCER1A; FcERI; Fcr-5; high affinity immunoglobulin epsilon receptor alpha-subunit; high affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; Iger01; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; RATIGER01; RP11-550P17.3
• Gene Symbols: FCER1A
• Product Type: Antibody