Feline Immunodeficiency Virus p24 gag Antibody (PAK3-2C1) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB100648020.025MG

Catalog No. NB017643

Description

Feline Immunodeficiency Virus p24 gag Monoclonal specifically detects Feline Immunodeficiency Virus p24 gag in Virus samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen.

Specifications

• Antigen: Feline Immunodeficiency Virus p24 gag
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Gene Alias: CA, Capsid protein p24, Feline immunodeficiency virus, Feline immunodeficiency virus capsid protein p24, Feline immunodeficiency virus p24, FIV capsid protein p24
• Immunogen: Made to Viral FELINE IMMUNODEFICIENCY VIRUS p24 gag
• Quantity: 0.025 mg
• Primary or Secondary: Primary
• Target Species: Virus
• Isotype: IgG1

• Applications: Immunohistochemistry (Frozen), ELISA, Immunofluorescence, Western Blot, Flow Cytometry, Immunocytochemistry
• Clone: PAK3-2C1
• Conjugate: Unconjugated
• Formulation: PBS (pH 7.2) with 0.05% Sodium Azide
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Test Specificity: NB100-64802 recognizes an immunodominant p24 epitope of feline immunodeficiency virus (FIV) p24 gag. The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. The specific binding epitope recognized by clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding. NB100-64802 detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49 and 52kDa under reducing conditions in Western blotting. NB100-64802 does not recognize Feline Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

For Research Use Only