Invitrogen™ Fentanyl Polyclonal Antibody

Sheep Polyclonal Antibody

Supplier:  Invitrogen™ PA175148

Catalog No. PA175148

Description

Working Concentration: 1/500. Sensitivity: 10 ng/mL Fentanyl produces 84% inhibition in a competitive ELISA employing Fentanyl polyclonal antibody.

Fentanyl is a synthetic, lipophilic phenylpiperidine opioid agonist with analgesic and anesthetic properties. Fentanyl selectively binds to the mu-receptor in the central nervous system (CNS) thereby mimicking the effects of endogenous opiates. Stimulation of the mu-subtype opioid receptor stimulates the exchange of GTP for GDP on the G-protein complex and subsequently inhibits adenylate cyclase. This results in a decrease in intracellular cAMP and leads to a reduction in the release of neurotransmitters such as substance P, GABA, dopamine, acetylcholine and noradrenaline. The analgesic effect of fentanyl is likely due to its metabolite morphine, which induces opening of G-protein-coupled inwardly rectifying potassium (GIRK) channels and blocks the opening of N-type voltage-gated calcium channels, thereby resulting in hyperpolarization and reduced neuronal excitability. [National Center for Biotechnology Information. PubChem Compound Database; CID=3345].

Specifications

• Antigen: Fentanyl
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: C22H28N2O; Duragesic; Fentanest; Fentanil; Phentanyl
• Immunogen: Fentanyl-BSA.
• Quantity: 1 mg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Chemical

• Applications: ELISA
• Concentration: 13.26 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Host Species: Sheep
• Purification Method: purified
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov