Learn More
Thermo Scientific™ 5-Fluoroorotic Acid
Description
5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).
Recommended for:
Detection of URA3 gene expression in yeast; Construction of yeast 2-hybrid libraries (1); Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mutations.
Note:
Low solubility in water. Solubility in dimethylsulfoxide is greater than 150mg/mL. Preparation of a 100mg/mL solution in dime-thylsulfoxide is recommended.

Specifications
Specifications
Chemical Name or Material | 5-Fluoroorotic Acid |
Product Type | 5-Fluoroorotic Acid |
Quantity | 1 g |
Form | Powder |
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.