Thermo Scientific™ GeneJET Plasmid Midiprep Kit

Description

Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified— >400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines

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Specifications

• Concentration: 400 ng/μL
• Column Type: Spin Column
• Format: Spin Column
• Isolation Technology: Silica Column
• Scale: Midi
• Sample Type: Bacteria
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
• Product Line: GeneJET
• Product Type: Plasmid MidiPrep Kit
• Quantity: 25 Preps
• Target: Plasmid DNA
• Test Time: 60 min.

For Research Use Only. Not for use in diagnostic procedures.