Thermo Scientific™ GeneJET Whole Blood Genomic DNA Purification Mini Kit

Description

Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.

GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Highlights
• Efficient—high yields of high molecular weight genomic DNA
• Fast—20 minute procedure following cell lysis
• Convenient—spin columns are capped and assembled with collection tubes
• Pure—DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
• Compatible—suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids

Applications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting Includes Proteinase K Solution Lysis Solution Wash Buffer WB I (concentrated) Wash Buffer II (concentrated) Elution Buffer GeneJET Purification Columns (pre-assembled with collection tubes) Collection tubes Detailed Protocol

Specifications

• Column Type: Spin Column
• Content And Storage: • 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening
  • 120 mL Lysis Solution; room temperature
  • 40 mL Wash Buffer I (concentrated); room temperature
  • 40 mL Wash Buffer II (concentrated); room temperature
  • 150 mL Elution Buffer; room temperature
  • 250 GeneJET Purification Columns with Collection Tubes; room temperature
  • 250 Collection Tubes; room temperature
  
  
• Isolation Technology: Silica Spin Column
• Scale: Mini
• Sample Type: Bone Marrow, Buffy Coat, Dried Blood Spots, Buccal Swab, Urine, Whole Blood
• Elution Volume: 50 to 200 μL
• Final Product Type: gDNA
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 250 Preps
• Shipping Condition: Room Temperature
• Test Time: 20 min. (After sample homogenization)
• Yield: ≤65 μg
• Starting Material Amount: Bone Marrow: ≤200 μL
  Buccal Swabs: 1
  Buffy Coat: 200 μL
  Dried Blood Spots: varies
  Urine: ≤4.5 mL
  Whole Blood: ≤500 μL

For Research Use Only. Not for use in diagnostic procedures.