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R&D Systems™ FerroOrange
SDP

Catalog No. RD10188013 Shop All Novus Products
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RD10188013 24 μg
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Catalog No. RD10188013 Supplier R&D Systems™ Supplier No. 8846/24U

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Description

A Tocris product, part of R&D Systems. Sensitive and cell-permeable fluorogenic probe for the detection of labile Fe(II) in live cells; suitable for high-throughput assays

Key information: FerroOrange (RhoNox-4) is a highly sensitive and robust fluorogenic probe for the detection of intracellular labile Fe(II). FerroOrange displays weak absorption and low fluorescence in the visible region, but becomes strongly fluorescent upon reaction with Fe(II).

Used for: detection of intracellular labile Fe(II).

Application: high-throughput assays for high content imaging of subcellular labile Fe(II) in live cells; fluorescent microscopy; studies on iron homeostasis and ferroptosis.

Properties and Photophysical Data: FerroOrange has excellent cell-membrane permeability and low cell toxicity. FerroOrange has no chelating ability. For the fluorescent form, excitation and emission maxima (λ) measured in 50 mM HEPES buffer (pH 7.4, 0.2% DMSO) are 545 nm and 575 nm, respectively; extinction coefficient ε = 73,500 M-1cm-1; quantum yield φ = 0.19.

Please refer to this protocol for further information on how to use this product.

Specifications

Chemical Name or Material 4-(tert-Butoxycarbonyl)-1-(3'-(4-(tert-butoxycarbonyl)piperazin-1-yl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6'-yl)piperazine 1-oxide
CAS 2711762-70-6
Quantity 24 μg
Product Line Tocris
Target Ion Indicators
Molecular Formula C38H44N4O8
Purity 90%
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