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  6. Fibroblasts/Epithelial cells Antibody (D7-FIB) - BSA Free, Novus Biologicals™

Fibroblasts/Epithelial cells Antibody (D7-FIB) - BSA Free, Novus Biologicals™
SDP

Catalog No. p-7200892 Shop All R&D Systems Products
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0.025 mg
0.1 mg
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Catalog No. Quantity
NB018256 0.025 mg
NB600777 0.1 mg
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Catalog No. NB018256 Supplier Novus Biologicals Supplier No. NB6007770.025MG
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Description

Mouse Monoclonal Antibody has been used in 2 publications

Fibroblasts/Epithelial cells Monoclonal specifically detects Fibroblasts/Epithelial cells in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative).

Specifications

Antigen Fibroblasts/Epithelial cells
Applications Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen)
Classification Monoclonal
Clone D7-FIB
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 1:50-1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:100, Immunohistochemistry-Paraffin (Negative)
Formulation Phosphate buffered saline with 0.09% Sodium Azide
Host Species Mouse
Immunogen Human foreskin fibroblasts
Purification Method Protein G purified
Quantity 0.025 mg
Regulatory Status RUO
Research Discipline Fibroblast Cell Markers
Primary or Secondary Primary
Test Specificity NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.
Target Species Human
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG2a
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For Research Use Only

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