Invitrogen™ Fibronectin Polyclonal Antibody

Catalog No.	Quantity
PA123693	500 μL

Catalog No. PA123693 Supplier Invitrogen™ Supplier No. PA123693

Description

Rabbit Polyclonal Antibody

This antibody does not cross-react with Fibrinogen or other serum proteins. Prolonged fixation in buffered formalin may destroy the epitope. Auto/Zyme predigestion is recommended when using this antibody with formalin-fixed paraffin-embedded tissue sections. Suggested positive controls for this product are human tonsil, liver, skin or kidney.

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

Specifications

• Antigen: Fibronectin
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide; pH 7.4
• Gene: FN1
• Gene Accession No.: P02751
• Gene Alias: Anastellin; CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; cumulus cell-specific fibronectin 1 transcript; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; E330027I09; ED-B; embryo-specific fibronectin 1 transcript; ferritin L subunit; ferritin L-chain; FIBNEC; fibronectin; fibronectin 1; fibronectin ED-A- region; fibronectin ED-B+ region; fibronectin variable region; FINC; FN; FN1; fn-1; FNZ; GFND; GFND2; LETS; LOW QUALITY PROTEIN: fibronectin; Migration stimulating factor (MSF); migration-stimulating factor; MSF; Ugl-Y1; Ugl-Y2; Ugl-Y3
• Gene Symbols: FN1
• Host Species: Rabbit
• Immunogen: Purified human Fibronectin from human plasma.
• Purification Method: Protein A
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2335, 397620
• Target Species: Human, Pig
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG