Invitrogen™ FICD Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA567147

Catalog No. PIPA567147

Description

Immunogen sequence: VKKVMSIPKGN SALRRVMEET YYHHIYHTVA IEGNTLTLSE IRHILETRYA VPGKSLEEQN EVIGMHAAMK YINTTLVSRI GSVTISD Highest antigen sequence identity to the following orthologs - mouse 97%, rat 93%.

Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083). [UniProt].

Specifications

• Antigen: FICD
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ficd
• Gene Alias: adenosine monophosphate-protein transferase FICD; AMPylator FICD; D5Ertd40e; De-AMPylase FICD; FIC domain containing; FIC domain protein adenylyltransferase; FIC domain-containing protein; fic S-phase protein cell division homolog; FICD; HIP13; HIP-13; huntingtin interacting protein 13; huntingtin interacting protein E; huntingtin interactor protein E; huntingtin yeast partner E; Huntingtin-interacting protein 13; huntingtin-interacting protein E; Hype; MGC5623; Protein adenylyltransferase FICD; protein adenylyltransferase FICD; adenosine monophosphate-protein transferase FICD; UNQ3041; UNQ3041/PRO9857
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11153
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.05 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9BVA6
• Gene Symbols: Ficd
• Immunogen: Recombinant Human FICD. Recombinant protein control fragment (Product #RP-105519).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG