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Novus Biologicals™ Flagellin Native Protein
SDP

Catalog No. NB171710 Shop All R&D Systems Products
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NB171710 10 μg
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Catalog No. NB171710 Supplier Novus Biologicals™ Supplier No. NBP318149
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Description

Native Protein

Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at 5-50ng/mL & in vivo at a starting concentration of 0.1-1ug/mouse.

Specifications

Accession Number P06179
Concentration LYOPH
For Use With (Application) Bioactivity, ELISA
Formulation Lyophilized from PBS
Quantity 10 μg
Storage Requirements Store at 4C short term. Aliquot and Store at -20°C long term. Avoid freeze-thaw cycles.
Endotoxin Concentration <0.01 EU/μg purified protein (LAL test; Lonza).
Reconstitution Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution)
Research Category Immunology, Innate Immunity
Purity or Quality Grade >95% pure by SDS-PAGE
Protein Flagellin
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