FLK-1 Rat anti-Mouse, BB700, Clone: Avas 12alpha1, BD Optibuild™

Catalog No.	Quantity
BDB742184	50 μg

Catalog No. BDB742184 Supplier BD Biosciences Supplier No. 742184

Description

Rat Monoclonal Antibody

The Avas 12α1 monoclonal antibody specifically binds to fetal liver kinase 1 (Flk-1) which is also known as CD309. Flk-1 is a receptor protein tyrosine kinase which is closely related to CD117 (c-kit) and CD140a (PDGF Receptor α chain) of the immunoglobulin superfamily. Flk-1, also known as VEGF Receptor-2 (VEGF-R2 or VEGFR2), is a receptor for vascular endothelial growth factor (VEGF). It is expressed, at the mRNA and protein levels, on distinct sets of mesoderm during gastrulation and on endothelial cells in embryonic and adult tissues. In vivo and in vitro studies indicate that Flk-1 is required for the embryonic development of vascular endothelial and hematopoietic cells.

The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5. This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.

Specifications

• Antigen: FLK-1
• Classification: Monoclonal
• Clone: Avas 12alpha1
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Blue 700 (BB700)
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: CD309, Fetal liver kinase 1, Kdr, VEGF Receptor-2, VEGFR-2, AVAS12,
• Host Species: Rat
• Immunogen: Mouse Flk-1 Recombinant Protein
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ