Molecular Probes™ Fluo-4, AM, cell permeant  

Fluo-4, AM, cell permeant

Manufacturer:  Molecular Probes™ F14201

Catalog No. F14201

Description

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. Fluo-3 has been used to image the spatial dynamics of Ca²⁺ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.

Calcium Indicator (AM Ester) Specifications:

Label (Ex/Em of Ca²⁺–bound form): Fluo-4 (494/506 nm)

Fluorescence intensity increase upon binding Ca²⁺: >100 fold

K_d for Ca²⁺ in buffer: ~335 nM

Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength

Using TPEN to Control Heavy Metal Cations
In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn²⁺, Zn²⁺, Pb²⁺) with substantially higher affinity than Ca²⁺. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.

More Choices for Fluorescent Calcium Indicators
We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca²⁺ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

For UV-excitable Ca²⁺ indicators, protein-based Ca²⁺ indicators, conjugates of Ca²⁺ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg²⁺, Zn²⁺) review Indicators for Ca²⁺, Mg²⁺, Zn²⁺ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

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Specifications

• Quantity: 10 x 50μg
• Storage Requirements: –5 to –30°C
• For Use With (Equipment): For confocal microscope, fluorescence microscope, fluorescent imager, hts reader, high content instrument, microplate reader
• Product Type: Fluo-4 AM
• Excitation Wavelength Range: 456nm

• Description: Fluo-4 AM, 10 x 50μg, Orange, Solid, 1096.95 Molecular Weight, C51H50F2N2O23 Molecular Formula, Cell-Permanent, Fluorescent Detection Method, –5 to –30°C Storage, ≥95% at 254nm HPLC Purity, Use with Confocal Microscope, Fluorescence
• Detection Method: Fluorescent
• For Use With (Application): For cell viability, proliferation and function, cellular imaging, pharma and biopharma, target and lead identification and validation
• Format: Solid
• Color: Orange

For Research Use Only. Not for use in diagnostic procedures.