Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Cell Function Reagents and Kits
  6. Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit 

Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit

Catalog No. F10472
Encompass_Preferred
Change view
Click to view available options
Quantity:
10 x 10 mL
1 x 100 mL
1 x 1 L
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
F10472 1 x 100 mL
F10471 10 x 10 mL
50-114-7553 1 x 1 L
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. F10472 Supplier Molecular Probes™ Supplier No. F10472
Only null left
Add to Cart
Edge
Add to Cart

Description

Fluo-4 Direct™ Calcium Assay Kit

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:

  1. Easily loads into cells
  2. Achieves a large assay window and
  3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple 'addition only' format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.

TRUSTED_SUSTAINABILITY

Specifications

Color Green
Content And Storage
  • 100 ml Fluo-4 Direct™ Calcium Assay Reagent (Component A)
  • 2 × 77 mg Probenecid (Component B)
  • 200 ml Fluo-4 Direct™ Calcium Assay Buffer (Component C)
  • Store at ≤-20°C. Dessicate and protect from light.
Detection Method Fluorescence
For Use With (Application) Calcium Assay
For Use With (Equipment) Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Product Type Calcium Assay Kit
Dye Type Fluorescent Dye-Based
Emission Visible
Form Powder
Modification Chemical
Product Line Molecular Probes
Quantity 1 x 100 mL
Shipping Condition Room Temperature
Sub Cellular Localization Nucleus, Organelles, Cytoplasm
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.