Molecular Probes™ Fluo-4, Pentapotassium Salt, cell impermeant

Description

Fluo-4, Pentapotassium Salt, cell impermeant

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. Fluo-3 has been used to image the spatial dynamics of Ca²⁺ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette, microinjection, or our Influx™ pinocytotic cell-loading reagent. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.

Calcium Indicator (Cell-Impermeant Salts) Specifications:

Label (Ex/Em of Ca²⁺–bound form): Fluo-4 (494/516 nm)

Fluorescence intensity increase upon binding Ca²⁺: >100 fold

K_d for Ca²⁺ in buffer: ∼335 nM

Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength

Using TPEN to Control Heavy Metal Cations
In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn²⁺, Zn²⁺, Pb²⁺) with substantially higher affinity than Ca²⁺. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.

More Choices for Fluorescent Calcium Indicators
We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca²⁺ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

For UV-excitable Ca²⁺ indicators, protein-based Ca²⁺ indicators, conjugates of Ca²⁺ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg²⁺, Zn²⁺) review Indicators for Ca²⁺, Mg²⁺, Zn²⁺ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
• Product Type: Calcium Indicator
• Dye Type: Fluorescent Dye-Based
• Quantity: 500 μg
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.