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Fluorescein (FITC) Monoclonal Antibody (8B9.C6.D3), Rockland™
SDP

Catalog No. 501990501
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1 mg
100 μg
25 μL
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Catalog No. Quantity
50-199-0501 1 mg
50-199-0500 100 μg
50-199-0502 25 μL
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Catalog No. 50-199-0501 Supplier Rockland Immunochemicals Supplier No. 200301096L
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Mouse Monoclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

Specifications

Antigen Fluorescein (FITC)
Applications ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization (ISH), Western Blot
Classification Monoclonal
Clone 8B9.C6.D3
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Gene Alias Fluorescein
Host Species Mouse
Immunogen Fluorescein conjugated to Mouse IgG
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Primary
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG2a κ
Target Molecule Chemical
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