Fluorescein (FITC) Polyclonal Antibody, Rockland™

Goat Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-105-7773	Rockland Immunochemicals 600101096S	25 μL
50-199-1546	Rockland Immunochemicals 600101096	1 mg

Description

Store vial at 4°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

Specifications

• Antigen: Fluorescein (FITC)
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Goat
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Primary or Secondary: Primary
• Product Type: Antibody

• Concentration: 1 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Alias: Fluorescein
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.