Fluorescein-SMRT ID2 Corepressor Peptide, 100µM 

Fluorescein-SMRT ID2 Corepressor Peptide, 100 µM

Catalog Number	Mfr. No.	Quantity
PV4423	LIFE TECHNOLOGIES PV4423	100μL

Description

LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

Contents and Storage:
LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100µM solutions in peptide-appropriate buffers. Store peptides at -20°C.

Specifications

• Common Name: Fluorescein - SMRT ID2 Corepressor Peptide
• For Use With (Application): Pharma and biopharma, target and lead identification and validation, drug discovery and development, nuclear receptor biology, nuclear receptor proteins, biochemical nuclear receptor assays, nuclear receptor coregulator interaction
• Product Type: Fluorescein - SMRT ID2 Corepressor Peptide
• Regulatory Status: For research use only. Not for use in diagnostic procedures
• Storage Buffer: 25mM HEPES (pH 7.5), 50% DMSO
• Validated Application: Pharma and biopharma, target and lead identification and validation

• Concentration: 100μm
• Molecular Weight: 2993Da
• Purity: ≥95% Chromatographic by HPLC
• Sequence: Fluorescein - HASTNMGLEAIIRKALMGKYDQW
• Storage Requirements: –5 to –30°C

For Research Use Only. Not for use in diagnostic procedures.