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Invitrogen™ FokI Polyclonal Antibody

Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA5119997
This item is not returnable.
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Description
Positive Control: Purified restriction enzyme FokI.
The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
Specifications
FokI | |
Polyclonal | |
Unconjugated | |
Fok1 | |
Synthetic peptide within C-terminal of Planomicrobium okeanokoites fokIR. | |
100 μL | |
Primary | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |
Western Blot | |
1 mg/mL | |
PBS with 0.2% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4 | |
Rabbit | |
Affinity chromatography | |
RUO | |
Bacteria | |
Antibody | |
IgG |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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