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Invitrogen™ FokI Polyclonal Antibody
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Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5119997

Catalog No. PIPA5119997


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Description

Description

Positive Control: Purified restriction enzyme FokI.

The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

FokI
Polyclonal
Unconjugated
Fok1
Synthetic peptide within C-terminal of Planomicrobium okeanokoites fokIR.
100 μL
Primary
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Liquid
Western Blot
1 mg/mL
PBS with 0.2% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
Rabbit
Affinity chromatography
RUO
Bacteria
Antibody
IgG
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