Invitrogen™ FOXO1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1804

Catalog No. PA1804

Description

PA1-804 detects FoxO1a in human and rat samples. PA1- 804 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~66 kDa protein representing FoxO1a in rat heart and lung protein extracts. The PA1- 804 immunogen is a synthetic peptide corresponding to residues D(633) N V L P N Q S F P H S V K T(647) of human FOXO1a. This sequence is completely conserved in mouse and rat. Fig. 1 shows a Western blot of FoxO1a in rat lung protein extract using PA1-804.

FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.

Specifications

• Antigen: FOXO1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: FOXO1
• Gene Alias: Afxh; AI876417; FKH1; FKHR; Fkhr1; forkhead box O1; forkhead box O1 a; forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); forkhead box protein O1; forkhead box protein O1; LOW QUALITY PROTEIN: forkhead box protein O1; forkhead box protein O1A; Forkhead box protein O1-A; Forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead protein FKHR; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; fox family transcription factor FoxO1a.1; FOX01A; foxhead box protein O1-A; foxo1; FOXO1A; FoxO1a.1; member of FKHR-subclass forkhead/winged helix family; zgc:153388
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2308, 84482
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: G3V7R4, Q12778
• Gene Symbols: FOXO1
• Immunogen: Synthetic peptide corresponding to residues D(633) N V L P N Q S F P H S V K T(647) of human FOXO1a.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov