Invitrogen™ FPGS Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527914

Catalog No. PIPA527914

Description

Recommended positive controls: Folylpolyglutamate synthase tansfected 293T cell. Predicted reactivity: Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Folylpoly-gamma-glutamate synthetase (FPGS) catalyzes the addition of several equivalents of glutamic acid to the gamma-carboxyl group in the side chain of folate cofactors and analogs. Folylpoly-gamma-glutamate synthetase has three functions in folate homeostasis in mammals: polyglutamation prevents efflux of folate cofactors from the cell, it increases the binding of folate cofactors to some of the enzymes of folate interconversion and biosynthesis, and it appears to allow the accumulation of folates in the mitochondria that are required for glycine synthesis. Folylpoly-gamma-glutamate synthetase is widely expressed in human tumors and is tightly linked either to proliferation or to a lack of differentiation. The cytotoxicity of both thymidylate synthase and purine inhibitors requires continued inhibition of target for greater than one generation time, so that the integrative function of FPGS adds considerably to the efficiency of folate antimetabolites.

Specifications

• Antigen: FPGS
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: FPGS
• Gene Alias: AA408187; folylpoly-gamma-glutamate synthetase; folylpolyglutamate synthase; folylpolyglutamate synthase, mitochondrial; folylpolyglutamyl synthetase; FPGS; Tetrahydrofolate synthase; tetrahydrofolylpolyglutamate synthase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2356
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q05932
• Gene Symbols: FPGS
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Folylpolyglutamate synthase. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG