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Invitrogen™ Fura-2, AM, cell permeant (1 mM Solution in Anhydrous DMSO)

Catalog No. F1225
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Catalog No. F1225 Supplier Invitrogen™ Supplier No. F1225
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Description

Fura-2, AM is a high affinity, intracellular calcium indicator that is ratiometric and UV light–excitable.

Fura-2, AM is a high affinity, intracellular calcium indicator that is ratiometric and UV light–excitable. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading and is also available in 1 mg amounts (Cat. No. F-1201) and in special packagaing (F-1221). Cell-impermeant pentapotassium (F-1200) and pentasodium (F-6799) salt forms of the dye are available.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1 mM
Content And Storage Store in freezer (-5°C to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Application) Cell Viability and Proliferation
For Use With (Equipment) Fluorescence Microscope
Product Type Fura-2
Dye Type Fluorescent Dye-Based
Quantity 1 mL
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).
I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

For Research Use Only. Not for use in diagnostic procedures.

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