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Invitrogen™ eBioscience™ Fura-2 AM Dye Non-distribution product as customer accommodation.

Imaging dye that binds to free Ca2+. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when altering excitation wavelengths is more practical than detecting multiple emission wavelengths.

Manufacturer:  Invitrogen™ 65085839

Catalog No. 50-169-58



  • Upon binding Ca2+, the excitation spectrum of Fura-2 shifts to shorter wavelengths between 300 and 400nm, while the peak emission remains steady around 510nm
  • The Kd of Fura-2 is highly dependent on pH, temperature, ionic strength and viscosity of the cytosol, thus great care should be taken when non-standard conditions are used
  • Molecular weight: 1001.86g/mol
  • Peak excitation: variable depending on the concentration of free Ca2+, between 300 and 400nm
  • Peak emission: 510nm
  • Reconstitute in high-quality, freshly opened DMSO. Once reconstituted, protect from light and store at -20°C. Avoid freeze-thawing


−20°C. Protect from light and moisture.
>99% as determined by HPLC.
Microscopy, Cell Labeling
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