Invitrogen™ G-CSF Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA129653

Catalog No. PIPA129653

Description

Neutralization:To yield one-half maximal inhibition (ND50) of the biological activity of mG-CSF (0.5 ng/mL), a 0.005-0.010 μg/mL of this antibody is required. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

G-CSF (Granulocyte colony stimulating factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukemia cells. G-CSF is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. G-CSF is highly conserved among species and has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors, neutrophilic granulocytes and certain carcinoma cell lines. Clinical use of G-CSF has been approved for several therapeutic applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet's syndrome and AIDS. Further, G-CSF has been shown to be a marker protein for different carcinomas such as bladder cancer and dysfunction of the protein has been linked to Kostmann Syndrome.

Specifications

• Antigen: G-CSF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Csf3
• Gene Alias: C17orf33; colony stimulating factor 3; colony stimulating factor 3 (granulocyte); CSF 3; CSF3; CSF3OS; Csfg; filgrastim; GCSF; G-CSF; Granulocyte colony-stimulating factor; granulocyte-colony stimulating factor; gsf3; lenograstim; MGC45931; MGI-IG; Pluripoietin; RP23-395E10.4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12985
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Neutralization, Western Blot, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.4
• Gene Accession No.: P09920
• Gene Symbols: Csf3
• Immunogen: Highly pure (>98%) recombinant mG-CSF (mouse Granulocyte Colony Stimulating Factor ).
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov