Invitrogen™ GABRA1 Monoclonal Antibody (N95/35), PE

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545488

Catalog No. PIMA545488

Description

1 μg/mL of MA5-45488 was sufficient for detection of alpha1 GABA receptor in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or -Alpha3.

Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function.

Specifications

• Antigen: GABRA1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: P14867, P62812, P62813
• Gene Symbols: GABRA1
• Immunogen: Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Microarray
• Clone: N95/35
• Conjugate: PE
• Gene: GABRA1
• Gene Alias: ECA4; EIEE19; EJM; EJM5; GABA alpha subunit; GABA(A) receptor subunit alpha-1; GABA(A) receptor, alpha 1; GABAA alpha 1; GABAAR alpha1; GABRA 1; GABRA1; Gabra-1; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1; gamma-aminobutyric acid A receptor alpha 1 variant 1; gamma-aminobutyric acid A receptor alpha 1 variant 2; gamma-aminobutyric acid A receptor alpha 1 variant 3; gamma-aminobutyric acid A receptor alpha 1 variant 4; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid receptor subunit alpha-1; gamma-aminobutyric acid receptor subunit alpha-1-like; gamma-aminobutyric acid type A receptor alpha1 subunit; gamma-aminobutyric acid type A receptor subunit alpha1; LOW QUALITY PROTEIN: gamma-aminobutyric acid receptor subunit alpha-1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 14394, 2554, 29705
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov