Invitrogen™ GABRA5 Monoclonal Antibody (N415/24), APC

Catalog No.	Quantity
PIMA545745	100 μg

Catalog No. PIMA545745

Supplier: Invitrogen™ MA545745

Description

Mouse Monoclonal Antibody

A 1:100 dilution of MA5-45745 was sufficient for detection of GABA-A R, Alpha5 in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data. This antibody was formerly sold as clone S415-24.

GABRA5 is a part of GABA, a major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.

Specifications

• Antigen: GABRA5
• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: N415/24
• Concentration: 1 mg/mL
• Conjugate: APC
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene: GABRA5
• Gene Accession No.: P19969, P31644, Q8BHJ7
• Gene Alias: A230018I05Rik; GABA(A) receptor; GABA(A) receptor subunit alpha-5; GABAA receptor alpha5 subunit; GABA-A receptor alpha-5 subunit; GABRA5; Gabra-5; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 5; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 5; gamma-aminobutyric acid A receptor, alpha 5; gamma-aminobutyric acid receptor subunit alpha-5; gamma-aminobutyric acid type A receptor alpha5 subunit; ligand-gated ion channel subunit
• Gene Symbols: GABRA5
• Host Species: Mouse
• Immunogen: Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 110886, 2558, 29707
• Target Species: Human, Mouse, Rat
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov