Invitrogen™ GAD67 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA585371

Catalog No. PIPA585371

Description

Keep as concentrated solution. Predicted reactivity: Mouse (96%), Rat (96%), Dog (99%), Cat (99%), Pig (99%), Chicken (91%), Chimpanzee (100%), Bovine (98%). Positive Control: SK-N-SH, mouse brain, rat brain, human GAD1-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Specifications

• Antigen: GAD67
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GAD1
• Gene Alias: 67 kDa glutamic acid decarboxylase; 67kDa glutamate decarboxylase 1; CPSQ1; EGK_04525; EP10; FLJ45882; GAD; GAD 1; GAD 67; GAD1; Gad-1; GAD25; GAD44; Gad67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 isoform GAD67; glutamate decarboxylase 1 variant GAD67NT; glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); glutamic acid decarboxylase; glutamic acid decarboxylase 1; glutamic acid decarboxylase isoform 67; OTTHUMP00000041055; SCP
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14415, 24379, 2571
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Western Blot
• Concentration: 0.17 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P18088, P48318, Q99259
• Gene Symbols: GAD1
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GAD67.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG