Invitrogen™ GAD67 Recombinant Rabbit Monoclonal Antibody (JM11-11)

Catalog No.	Quantity
PIMA532624	100 μL

Catalog No. PIMA532624

Supplier: Invitrogen™ MA532624

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Specifications

• Antigen: GAD67
• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Recombinant Monoclonal
• Clone: JM11-11
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene: GAD1
• Gene Accession No.: P18088, P48318, Q99259
• Gene Alias: 67 kDa glutamic acid decarboxylase; 67kDa glutamate decarboxylase 1; CPSQ1; EGK_04525; EP10; FLJ45882; GAD; GAD 1; GAD 67; GAD1; Gad-1; GAD25; GAD44; Gad67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 isoform GAD67; glutamate decarboxylase 1 variant GAD67NT; glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); glutamic acid decarboxylase; glutamic acid decarboxylase 1; glutamic acid decarboxylase isoform 67; OTTHUMP00000041055; SCP
• Gene Symbols: GAD1
• Host Species: Rabbit
• Immunogen: Synthetic peptide within Human GAD67 aa 60-99.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 14415, 24379, 2571
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG