Invitrogen™ GADD45B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143903

Catalog No. PIPA5143903

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human MCF-7 whole cell. IHC: human placenta tissue, human spleen tissue, human renal cell carcinoma tissue, human tonsil tissue. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cellcycle progression issubject to arrest at G1 and G2 checkpointsin response to DNA damage, presumablyto allow time for DNA repair prior to entryinto S andMphase, respectively. The p53 tumorsuppressor isrequired for one such G1 checkpoint and functionsto upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and inhibits entry ofcellsinto S phase. Thus, it has been suggested that GADD 45 mayserve as a link between the p53-dependentcellcycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45 beta and GADD 45 gamma, have been shown to be induced by environmentalstresses. GADD 45 beta and GADD 45 gamma are thought to induce p38/JNK activation via MEKK4 activation.

Specifications

• Antigen: GADD45B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GADD45B
• Gene Alias: AI323528; DKFZP566B133; Gadd45b; GADD45BETA; growth arrest and DNA damage inducible beta; growth arrest and DNA damage-inducible protein GADD45 beta; growth arrest and DNA-damage-inducible 45 beta; growth arrest and DNA-damage-inducible protein GADD45 beta; growth arrest and DNA-damage-inducible, beta; MYD118; myeloid differentiation primary response; myeloid differentiation primary response gene 118; myeloid differentiation primary response protein MyD118; Negative growth regulatory protein MyD118; Unknown (protein for MGC:138134)
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4616
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: O75293
• Gene Symbols: GADD45B
• Immunogen: A synthetic peptide corresponding to a sequence of human GADD45B (EELLVAAQRQDRLTVGVYE).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG