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Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells Non-distribution product as customer accommodation.

The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates. Sensitive The chemiluminescent assay exhibits over three o

Manufacturer:  Invitrogen™ T1014

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Catalog No. T1014


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Includes: Lysis solution, substrate, enhancer, and required buffer

Description

Description

  • Glow assay kinetics provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used
  • Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
  • Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
  • Assay sensitivity is 100- to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
  • Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
  • Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
  • Assay can be completed in about one hour, providing fast assay turnaround
Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
 
Versatile
The Galacto-Star system includes Galacton-Star chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.
 
Long-Lasting Signal
Light emission from the Galacto-Star assay reaches maximum in 60 to 90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.
 
Specifications
  • Assay Type: β-Gal (lacZ) Assay
  • Compatible Cells: Mammalian Cells
  • Detection Method: Chemiluminescent
  • For Use With Equipment: Luminometer (Microplate)
  • Form: Liquid
  • Format: 96-well plate
  • High Throughput Compatibility: High Throughput-Compatible
  • Readout: End Point
  • Sample Type (Specific): Cell Cultures
  • Substrate: Galacton-Star
  • Substrate Properties: Chemical Substrate
  • Substrate Type: Beta-Gal Substrate
  • Target Enzyme: Beta-Galactosidase
Specifications

Specifications

Reporter Gene Assays
96-well plate
Detection of β-galactosidase in mammalian cell lysates
1,800 assays
Enhanced Chemiluminescence
Lysis solution, substrate, enhancer, and required buffer
Luminometer or scintillation counter
Store at 2° to 8°C
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For Research Use Only. Not for use in diagnostic procedures.