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Invitrogen™ Mouse Galectin 1 ELISA Kit

Catalog No. p-7230788
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests

ELISA

The Mouse Galectin-1 (LGALS1) ELISA quantitates Ms Galectin-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Galectin-1. Principle of the method The Mouse Galectin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P16045
Assay Range 16.38 to 4000 pg/mL
Assay Sensitivity 12 pg/mL
Calibration Range 2 to 10 fold
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity The antibody pair provided in this kit recognizes mouse Galectin-1.
Sufficient For 96 Assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 16852
Gene Symbol AA410090,Gal-1,Galbp,L-14.5,L14,Lect14,galectin-1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Apoptosis
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym AA410090, Gal-1, Galbp, L-14.5, L14, Lect14, galectin-1
Target Galectin 1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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