Invitrogen™ Galectin 1 Mouse ELISA Kit 

Sandwich ELISA Kit

Catalog Number	Mfr. No.	Quantity
EMLGALS1	Invitrogen™ EMLGALS1	96 Tests

Description

The Mouse Galectin-1 (LGALS1) ELISA quantitates Ms Galectin-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Galectin-1. Principle of the method The Mouse Galectin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

Specifications

• Storage Requirements: 2°C to 8°C
• Assay Range: 16.38-4000 pg/mL
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
• Intraassay CV: <10%

• Assay Sensitivity: 12 pg/mL
• Test Time: 1 hr. 20 min.
• Interassay CV: <12%