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Invitrogen™ Galectin 3 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P246BT500UG

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Catalog No. 50-271-8574


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Description

Description

AA Sequence of recombinant protein: ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-3. Anti-Human Galectin-3-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hGalectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Galectin-3 (500-P246) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGalectin-3. Western Blot: To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGalectin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P246BT-1MG will be provided as 2 x 500 μg

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Galectin 3
Polyclonal
Biotin
LGALS3
35 kDa lectin; advanced glycation end-product receptor 3; AGE-R3; beta-galactosides-binding lectin; carbohydrate-binding protein 35; CBP 35; CBP30; CBP-30; CBP35; epsilon BP; gal3; gal-3; galactose-specific lectin 3; Galactoside-binding protein; GALBP; galectin 3; Galectin3; galectin-3; GALIG; GBP; IgE binding protein; igE-binding protein; L-29; L31; L-31; L-34; L-34 galactoside-binding lectin; Laminin-binding protein; lectin galactoside-binding soluble 3; lectin galactoside-binding soluble 3 protein; lectin L-29; lectin, galactose binding, soluble 3; lectin, galactoside binding soluble 3; lectin, galactoside-binding, soluble, 3; lectin, galactoside-binding, soluble, 3 (galectin 3); LGALS2; Lgals3; MAC2; Mac-2; Mac-2 antigen
Rabbit
Antigen affinity chromatography
RUO
3958
-20°C
Lyophilized
ELISA, Western Blot
0.1-1.0 mg/mL
PBS with no preservative
P17931
LGALS3
E.coli-derived Recombinant Human Galectin-3.
500 μg
Primary
Human
Antibody
IgG
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