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PeproTech Galectin 3 Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P24650UG
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500P24650UG 50 μg
500P246100 100 μg
500P2461MG 1 mg
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Catalog No. 500P24650UG Supplier PeproTech Supplier No. 500P24650UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-3. Anti-Human Galectin-3-specific antibody was purified by affinity chromatography employing an immobilized Human Galectin-3 matrix. Sandwich ELISA: To detect Human Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Galectin-3 (500-P246Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Galectin-3. Western Blot: To detect Human Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant HUman Galectin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Galectin 3
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene LGALS3
Gene Accession No. P17931
Gene Alias 35 kDa lectin; advanced glycation end-product receptor 3; AGE-R3; beta-galactosides-binding lectin; carbohydrate-binding protein 35; CBP 35; CBP30; CBP-30; CBP35; epsilon BP; gal3; gal-3; galactose-specific lectin 3; Galactoside-binding protein; GALBP; galectin 3; Galectin3; galectin-3; GALIG; GBP; IgE binding protein; igE-binding protein; L-29; L31; L-31; L-34; L-34 galactoside-binding lectin; Laminin-binding protein; lectin galactoside-binding soluble 3; lectin galactoside-binding soluble 3 protein; lectin L-29; lectin, galactose binding, soluble 3; lectin, galactoside binding soluble 3; lectin, galactoside-binding, soluble, 3; lectin, galactoside-binding, soluble, 3 (galectin 3); LGALS2; Lgals3; MAC2; Mac-2; Mac-2 antigen
Gene Symbols LGALS3
Host Species Rabbit
Immunogen E.coli-derived Recombinant Human Galectin-3.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3958
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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