GAPDH Antibody (13H12) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 29 publications

Supplier:  Novus Biologicals NBP227103

Catalog No. NBP227103

Description

GAPDH Monoclonal specifically detects GAPDH in Human, Mouse, Rat, Drosophila, Monkey, Primate, Sheep samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: GAPDH
• Classification: Monoclonal
• Concentration: 1.0 mg/ml
• Dilution: Western Blot 0.25 - 1 ug/ml, Simple Western 1:25, Immunohistochemistry 5 ug/ml, Immunocytochemistry/Immunofluorescence 1:10, Immunohistochemistry-Paraffin 5 ug/ml
• Gene Alias: aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
• Host Species: Mouse
• Molecular Weight of Antigen: 36 kDa
• Quantity: 0.1 mg
• Research Discipline: Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
• Gene ID (Entrez): 2597
• Target Species: Human, Mouse, Rat, Drosophila, Primate, Sheep
• Form: Purified

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 13H12
• Conjugate: Unconjugated
• Gene Accession No.: P04406
• Gene Symbols: GAPDH
• Immunogen: Amino acids between 275 and 325 of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen for this GAPDH antibody.
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Reacts with Human, Primates/Monkey, Mouse and Drosophila. Based upon 91% sequence similarity with immunogen, this antibody is predicted to react with Guinea Pig, Sheep, Squirrel, Porcine/Pig, Ferret, Canine/Dog/Cat, Bovine, Reptile / Rattlesnake and several other species. Immunogen shows 82% similarity to Xenopus and Zebrafish.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only