Invitrogen™ GAPDHS Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA587928

Catalog No. PIPA587928

Description

Immunogen sequence: ASDHISAGAQ RVVISAPSPD APMFVMGVNE NDYNPGSMNI VSNASCTTNC LAPLAKVIHE RFGIVEGLMT TVHSYTATQK TVDGPSRKAW RDGRGAHQNI IPASTGAAKA VTKVIPELKG KLTGMAFRVP TPDVSVVDLT CRLAQPAPYS AIKEAVKAAA KGPMAGILAY TEDEVVSTDF LGDTHSSIFD AKAGIALNDN FVKLISWYDN EYGYSHRVVD LLRYMFSRDK; Positive Samples: HT-1080, U-251MG, SW620, MCF-7, Mouse liver, Rat testis; Cellular Location: Cytoplasm.

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

Specifications

• Antigen: GAPDHS
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GAPDHS
• Gene Alias: epididymis secretory protein Li 278; GAPD2; GAPDH S; GAPDH2; gapdh-2; GAPDHS; GAPDS; Gapd-s; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HEL-S-278; HSD35; HSD-35; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14447, 26330, 66020
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 4.49 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O14556, Q64467, Q9ESV6
• Gene Symbols: GAPDHS
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 179-408 of human GAPDHS (NP_0551791).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG