GARP Mouse anti-Human, BUV395, Clone: 7B11 (also known as CMSSC-7B11), BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 742804

Catalog No. BDB742804

Description

The 7B11 (also known as CMSSC-7B11) monoclonal antibody specifically binds to human GARP (Glycoprotein A repetitions predominant). The LRRC32 /(Leucine rich repeat containing 32) gene encodes the 662 amino acid-residue, 80 kDa transmembrane GARP glycoprotein that has an extracellular region composed primarily of 20 leucine-rich repeats. GARP is specifically expressed on Treg cells activated through the T cell receptor (TCR). Ectopic expression of GARP in human naοve T cells inhibited their proliferation and cytokine secretion upon TCR activation. Remarkably, GARP over-expression in naοve T cells induced expression of FoxP3 and endowed them with a partial suppressive function. The extracellular, but not the cytoplasmic region, of GARP was necessary for these functions. GARP serves as a receptor for latent TGF-beta which may play a role in the suppressive action of Treg cells. GARP is also expressed on platelets and other tissues, however the function on these cells is not known.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: GARP
• Clone: 7B11 (also known as CMSSC-7B11)
• Conjugate: Brilliant Ultraviolet 395
• Gene Alias: LRRC32, Garpin, Glycoprotein A repetitions predominant
• Immunogen: Recombinant Human LRRC32
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG2b κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov