GAS2L1, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
ABN226125UG	MilliporeSigma ABN226125UG	25 μg
ABN2261100	MilliporeSigma ABN2261100UG	100 μg

Description

Anti-GAS2L1, Cat. No. ABN2261, is a highly specific rabbit polyclonal antibody that targets GAS2-like protein 1 (Gas2L1) and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

GAS2-like protein 1 (UniProt: Q99501-1; also known as GAS2-related protein on chromosome 22, Growth arrest-specific protein 2-like 1, GAS2L1) is encoded by the GAS2L1 (also known as GAR22) gene (Gene ID: 10634) in human. GAS2L1 belongs to the growth-arrest-specific 2 (GAS2) family, whose members mediate the crosstalk between filamentous actin (F-actin) and microtubules. Three members of this family, known as GAS2L1, GAS2L2, and GAS2L3, are shown to be differentially involved in mediating the crosstalk between F-actin and microtubules. GAS2L1 is expressed in testis and brain, GAS2L2 is exclusively expressed in skeletal muscle, and GAS2L3 is found in many different cell types. Although they all localize to actin and microtubules, only the exogenous expression of GAD2L1 and GAS2L2 affect microtubule stability, dynamics, and guidance along actin stress fibers. GAS2L1 is reported to associate with the proximal end of mature centrioles and participates in centriole dynamics and centrosome disjunction. Loss of GAS2L1 can inhibit centrosome disjunction in the G2 phase. On the other hand, overexpression of GAS2L1 can induce premature centrosome separation, which also requires its association with actin, microtubules, and the microtubule end-binding proteins. (Ref.: Stroud MJ et al. (2014). J. Cell Sci. 127(12); 2672-2682; AU, FKC et al. (2017). Dev. Cell 40(1); 81-94).

Specifications

• Antigen: GAS2L1
• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: Neuroscience
• Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 10% glycerol.
• Gene ID (Entrez): NP_689422
• Immunogen: GST-tagged recombinant fragment corresponding to 188 amino acids from the C-terminal region of human GAS2-Like protein 1.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Molecular Weight of Antigen: ∽78 kDa observed; 72.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

• Dilution: Western Blotting Analysis: A representative lot detected GAS2L1 in Western Blotting applications (Au, F.K., et. al. (2017). Dev Cell. 40(1):81-94).Immunoprecipitation Analysis: 1μg from a representative lot detected GAS2L1 in U2OS cell lysate (Courtesy of Dr Robert Z. Qi and Dr. Franco K.C. Au at Hong Kong University of Science and Technology).Immunofluorescence Analysis: A representative lot detected GAS2L1 in Immunofluorescence applications (Au, F.K., et. al. (2017). Dev Cell. 40(1):81-94).Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAS2L1 in lysate from HeLa cells, but not in lysate from GAS2L1 knockout cells (Courtesy of Dr Robert Z. Qi and Dr. Franco K.C. Au at Hong Kong University of Science and Technology).
• Classification: Polyclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: GAS2-like protein 1;GAS2-related protein on chromosome 22;Growth arrest-specific protein 2-like 1
• Gene Symbols: GAS2L1;GAR22
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: This rabbit polyclonal antibody GAS2-like protein 1 (GAS2L1) in human cells. It targets an epitope with in 188 amino acids from the C-terminal half.