Invitrogen™ GATA3 Recombinant Rabbit Monoclonal Antibody (BLR121H)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA544431	Invitrogen™ MA544431	100 μL
PIMA544432	Invitrogen™ MA544432	10 μL

Description

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

Specifications

• Antigen: GATA3
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: BBS with 0.09% sodium azide; pH 8.2
• Gene Accession No.: P23771
• Gene ID (Entrez): 2625
• Immunogen: Peptide representing a region between residue 150 and 200 of Human trans-acting T-cell-specific transcription factor GATA3.
• Product Type: Antibody

• Clone: BLR121H
• Content And Storage: 4°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: GATA3
• Gene Alias: EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
• Gene Symbols: GATA3
• Primary or Secondary: Primary
• Purification Method: Purified