Invitrogen™ GCAP1 Monoclonal Antibody (G2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1724

Catalog No. MA1724

Description

MA1-724 detects GCAP-1 from human, non-human primate and bovine samples. This antibody is specific for GCAP-1 and does not react with other isotypes. MA1-724 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~23 kDa protein representing GCAP-1 protein on bovine rod outer segment membranes. The MA1-724 immunogen is bacterial expressed full length GCAP-1.

Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

Specifications

• Antigen: GCAP1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1 mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P43080, P46065
• Gene Symbols: Guca1a
• Immunogen: Bacterial expressed full length GCAP-1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Monkey, Bovine
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot
• Clone: G2
• Conjugate: Unconjugated
• Gene: Guca1a
• Gene Alias: C6orf131; COD3; cone dystrophy 3; CORD14; GC-A; GCAP; GCAP 1; GCAP1; Guanylate cyclase activator 1A; guanylate cyclase activator 1a (retina); guanylate cyclase-activating protein, photoreceptor 1; guanylin 1, retina; guanylyl cyclase-activating protein; guanylyl cyclase-activating protein 1; GUCA; GUCA1; GUCA1A; mGCAP1
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 282243, 2978
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid