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Invitrogen™ GCAP1 Monoclonal Antibody (G2)
Mouse Monoclonal Antibody
Supplier: Invitrogen™ MA1724
Description
MA1-724 detects GCAP-1 from human, non-human primate and bovine samples. This antibody is specific for GCAP-1 and does not react with other isotypes. MA1-724 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~23 kDa protein representing GCAP-1 protein on bovine rod outer segment membranes. The MA1-724 immunogen is bacterial expressed full length GCAP-1.
Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.
Specifications
GCAP1 | |
Monoclonal | |
1 mg/mL | |
PBS with 1 mg/mL BSA and 0.05% sodium azide | |
P43080, P46065 | |
Guca1a | |
Bacterial expressed full length GCAP-1. | |
100 μg | |
Primary | |
Human, Monkey, Bovine | |
Antibody | |
IgG2a |
Western Blot | |
G2 | |
Unconjugated | |
Guca1a | |
C6orf131; COD3; cone dystrophy 3; CORD14; GC-A; GCAP; GCAP 1; GCAP1; Guanylate cyclase activator 1A; guanylate cyclase activator 1a (retina); guanylate cyclase-activating protein, photoreceptor 1; guanylin 1, retina; guanylyl cyclase-activating protein; guanylyl cyclase-activating protein 1; GUCA; GUCA1; GUCA1A; mGCAP1 | |
Mouse | |
Protein A | |
RUO | |
282243, 2978 | |
-20°C, Avoid Freeze/Thaw Cycles | |
Liquid |
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