Invitrogen™ GCAP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5140166

Catalog No. PIPA5140166

Description

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. Immunogen sequence: MGNVMEGKSV EELSSTECHQ WYKKFMTECP SGQLTLYEFR QFFGLKNLSP SASQYVEQMF ETFDFNKDGY.

Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

Specifications

• Antigen: GCAP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Guca1a
• Gene Alias: C6orf131; COD3; cone dystrophy 3; CORD14; GC-A; GCAP; GCAP 1; GCAP1; Guanylate cyclase activator 1A; guanylate cyclase activator 1a (retina); guanylate cyclase-activating protein, photoreceptor 1; guanylin 1, retina; guanylyl cyclase-activating protein; guanylyl cyclase-activating protein 1; GUCA; GUCA1; GUCA1A; mGCAP1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2978
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P43080
• Gene Symbols: Guca1a
• Immunogen: Recombinant protein corresponding to Human GUCA1A. Recombinant protein control fragment (Product #RP-109449).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG