GCP2, Mouse anti-Mouse, Chicken, Human, Frog, Fish, Clone: GCP2-02, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABT132225UG

Catalog No. MABT132225

Description

Anti-GCP2, clone GCP2-02, Cat. No. MABT1322, is a mouse monoclonal antibody that detects Gamma-tubulin complex component 2 (CGP2) and has been tested for use in ELISA, Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Gamma-tubulin complex component 2 (UniProt: Q921G8; also known as GCP2) is encoded by the Tubgcp2 (also known as Gcp2) gene (Gene ID: 74237) in murine species. Gamma-tubulin complex that is essential for microtubule nucleation at the centrosome is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6. GCPs are characterized by sequence homology in two specific regions known as the grip1 and grip2 motifs. GPCs associate with gamma-tubulin to form a V-shaped subcomplex known as gamma-tubulin small complex. In this complex GCPs 2 and 3 interact via their N-terminal domain and form the arms of the V. The two-distal part of the V (C-terminal domains) bind to gamma-tubulin. The gamma-tubulin complex serves as a template for polarized growth and regulation of microtubules in eukaryotes. Significantly higher expression of both GPC2 and 3 have been reported in glioblastoma cells and they are concentrated I the centrosomes in interphase glioblastoma cells. Depletion of GPC2 and 3 causes accumulation of cells in G2/M and result in mitotic delay. (Ref.: Farache, D., et al. (2016). J. Biol. Chem. 291(44); 23112-25; Draberova, E., et al. (2015). J. Neuropathol. Exp. Neurol. 74(7); 723-42).

Specifications

• Antigen: GCP2
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: Tubgcp2;Gcp2
• Immunogen: GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2.
• Quantity: 25 μg
• Research Discipline: Cell Structure
• Gene ID (Entrez): NP_001272936.1
• Target Species: Mouse, Chicken, Human, Frog, Fish
• Form: Purified

• Applications: ELISA, Immunocytochemistry, Immunomicroscopy, Immunoprecipitation, Western Blot
• Clone: GCP2-02
• Dilution: Western Blotting Analysis: 1μg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunoprecipitation Analysis: A represnetative lot immunoprecipitated GCP2 in mouse bone-marrow mast cells (Courtesy of Pavel Draber, Ph.D., Institute of Molecular Genetics of the ASCR, v.v.i., Prague, Czech Republic).
• Gene Alias: Gamma-tubulin complex component 2
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope within 12 amino acids from the N-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ