Cytiva GST Gene Fusion System pGEX Vectors

Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST

Catalog Number	Mfr. No.	Kit Contents
45-002-271	Cytiva 28954549	4T-1
45-002-276	Cytiva 28954650	6P-2
45-002-277	Cytiva 28954651	6P-3
45-002-275	Cytiva 28954648	6P-1
45-002-278	Cytiva 28954653	2T
45-002-282	Cytiva 28954552	4T-3

Description

• Offers a tac promoter for chemically inducible, high-level expression of GST-tagged recombinant proteins
• Offers an internal laq1_q gene for use in any E. coli host
• With PreScission™ Protease, Thrombin, or Factor Xa recognition sites for cleaving the target protein from the fusion product
• The vectors provide all three translational reading frames beginning with the EcoRI restriction site
• Can use a wide variety of E. coli host strains cloning and expression

• pGEX-1lT EcoRI/BAP is an EcoR1 pre cut vector, phosphatase treated to prevent self ligation, ready for gene insertion
• pGEX-2TK will introduce a specific kinase site allowing the fusion protein to be labelled with [γ-32P]ATP using protein kinase

Specifications

• Quantity: 25 μg
• For Use With (Application): Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schisto soma japonicum GST
• Type: pGEX Vector