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GE Healthcare HiPrep™ IMAC FF 16/10 Column Available on GSA/VA Contract for Federal Government customers only.

For optimizing purification of histidine-tagged proteins at laboratory scale

Manufacturer:  GE Healthcare 28936552

Catalog No. 45-002-072


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Description

Description

IMAC Sepharose™ 6 Fast Flow is prepacked in 20mL HiPrep columns for greater scale up. The medium consists of 90µm beads of highly crosslinked agarose, to which a chelating group has been covalently coupled. This group may be charged with suitable metal ions, specific for your target protein.The bead size enables high flow properties; low metal ion leakage retains activity of the purified protein.

Specifications:
IMAC Sepharose 6 Fast Flow

  • Matrix: highly crosslinked agarose, 6%
  • Average particle size: 90µm
  • Binding capacity (protein/ion dependent): approx. 40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Compatibility: stable in all commonly used buffers
  • Chemical stability (uncharged medium) one week, 40°C: 0.01 M HCl, 0.1 M NaOH; 12 hr.: 1M NaOH, 70% acetic acid; 1 hr. tested 2% SDS; 30 min. tested 30% 2-propanol
  • Stability (pH, w/ uncharged medium w/ metal ion): 3 to 12 (long-term), 2 to 14 (short-term)
  • Storage/Temperature: 20% ethanol; 4° to 30°C

HiPrep Prepacked Columns

  • Volume: 20mL
  • Dimensions: 1.6 x 10cm
  • Max. pressure during operation: 1.5bar (0.15MPa, 22psi)
  • Hardware pressure limit: 5bar (0.5MPa, 73psi)
  • Recommend flowrate (H2O at room temp.): 2 to 10mL/min.
  • This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

    Specifications

    Specifications

    0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
    0.62 in.
    HiTrap IMAC Sepharose 6 FF column
    IMAC and subsequent purification of Polyhistidine tagged Proteins
    100mm
    Polypropylene
    3 to 12
    6% Cross-linked Agarose
    20mL
    16mm
    1 x 20mL
    3.93 in.
    <10mL/min.
    1.5 bar
    45 to 165μm
    4° to 30°C
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