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GE Healthcare HiPrep™ IMAC FF 16/10 Column Available on GSA/VA Contract for Federal Government customers only.

For optimizing purification of histidine-tagged proteins at laboratory scale

Manufacturer:  GE Healthcare 28936552

Catalog No. 45-002-072

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IMAC Sepharose™ 6 Fast Flow is prepacked in 20mL HiPrep columns for greater scale up. The medium consists of 90µm beads of highly crosslinked agarose, to which a chelating group has been covalently coupled. This group may be charged with suitable metal ions, specific for your target protein.The bead size enables high flow properties; low metal ion leakage retains activity of the purified protein.

IMAC Sepharose 6 Fast Flow

  • Matrix: highly crosslinked agarose, 6%
  • Average particle size: 90µm
  • Binding capacity (protein/ion dependent): approx. 40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Compatibility: stable in all commonly used buffers
  • Chemical stability (uncharged medium) one week, 40°C: 0.01 M HCl, 0.1 M NaOH; 12 hr.: 1M NaOH, 70% acetic acid; 1 hr. tested 2% SDS; 30 min. tested 30% 2-propanol
  • Stability (pH, w/ uncharged medium w/ metal ion): 3 to 12 (long-term), 2 to 14 (short-term)
  • Storage/Temperature: 20% ethanol; 4° to 30°C

HiPrep Prepacked Columns

  • Volume: 20mL
  • Dimensions: 1.6 x 10cm
  • Max. pressure during operation: 1.5bar (0.15MPa, 22psi)
  • Hardware pressure limit: 5bar (0.5MPa, 73psi)
  • Recommend flowrate (H2O at room temp.): 2 to 10mL/min.
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    0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
    0.62 in.
    HiTrap IMAC Sepharose 6 FF column
    IMAC and subsequent purification of Polyhistidine tagged Proteins
    3 to 12
    6% Cross-linked Agarose
    1 x 20mL
    3.93 in.
    1.5 bar
    45 to 165μm
    4° to 30°C
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