GE Healthcare HisTrap™ FF Crude Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Manufacturer: GE Healthcare 11000458

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Catalog No. 45-000-000

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    Bed Volume 1mL
    Includes Sodium Phosphate-buffered Saline, pH 7.4 and 0.5X concentration of proprietary Y-PER reagent additives
    Material Polypropylene
    Storage Requirements 4° to 30°C
    Support Type 6% Cross-linked Agarose
    Diameter (English) Inner 0.2 in.
    Packaging Quantity 5 x 1mL
    pH Range 3 to 12
    Particle Size 45 to 165μm
    Product Type HisTrap FF Crude Column
    Pressure 3 bar
    Quantity 60mL
    Diameter (Metric) Inner 7mm
    Flow Rate <4mL/min.

    • Prepacked with Ni Sepharose™ 6 Fast Flow media
    • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
    • Maximum pressure: 3 bar
    • Column material: polypropylene
    • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
    • 1 and 5mL columns

    • Matrix: 6% cross-linked agarose
    • Average particle size: 90µm
    • pH stability working range: 3 to 12

    1mL columns

    • Bed dimensions: 7 x 25mm
    • Flow velocity: <4mL/min.

    5mL columns
    • Bed dimensions: 16 x 25mm
    • Flow velocity: <20mL/min.

  • Description & Specifications

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