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GE Healthcare HisTrap™ HP Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Manufacturer:  GE Healthcare 17524701

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Catalog No. 45-000-323


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Description

Description

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34µm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.

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Specifications

Specifications

1mL
7mm
4 mL/min (1 mL), 5 mL/min (5ml)
5 x 1 mL
HisTrap HP, 5 x 1 mL
5 x 1mL
6% Highly Cross-linked Agarose
0.2 in.
HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Polypropylene
3 to 12
5 bar [0.5 MPa] (70 psi)
4° to 30°C
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